Scientific Papers

2019

• Brígido, C.; Menéndez, E.; Paço, A.; Glick, B.R.; Belo, A.; Félix, M.R.; Oliveira, S.; Carvalho, M. (2019) Mediterranean Native Leguminous Plants: A Reservoir of Endophytic Bacteria with Potential to Enhance Chickpea Growth under Stress Conditions. Microorganisms  , 7  , 392. https://www.mdpi.com/2076-2607/7/10/392
• Brígido, C., Singh, S., Menéndez, E., Tavares, M. J., Glick, B. R., Félix, M. d. R., Oliveira, S., and Carvalho, M. (2019). Diversity and Functionality of Culturable Endophytic Bacterial Communities in Chickpea Plants. Plants 8(2), 42; https://doi.org/10.3390/plants8020042 .
• da-Silva, J.R., Menéndez, E., Eliziário, F., Mateos, P.F., Alexandre, A. and Oliveira, S. (2019). Heterologous expression of nifA  or nodD  genes improves chickpea-Mesorhizobium  symbiotic performance. Plant and Soil 436 (1-2), 607-621. https://doi.org/10.1007/s11104-019-03950-0 .
• Paço, A., da-Silva, J.R., Eliziário, F., Brígido, C., Oliveira, S. and Alexandre, A. (2019) traG  Gene Is Conserved across Mesorhizobium  spp. Able to Nodulate the Same Host Plant and Expressed in Response to Root Exudates. BioMed Research International, vol. 2019, Article ID 3715271, 13 pages. https://doi.org/10.1155/2019/3715271 .

2018

• Serrano J., Shahidian S., Silva J. M. and Carvalho, M. (2018) A Holistic Approach to the evaluation of the montado ecosystem using proximal sensors. Sensors, 18 570, 1-24.http://www.mdpi.com/1424-8220/18/2/570
• Serrano J., Sales-Baptista E., Shahidian S., Silva J. M., Oliveira I. F., Castro J. L., Pereira A., Abreu M. C., and Carvalho M. (2018) Proximal sensors for monitoring seasonal changes of feeding sites selected by grazing ewes. Agroforest Syst., https://doi.org/10.1007/s10457-018-0219-5.https://link.springer.com/article/10.1007/s10457-018-0219-5

2017

• Menéndez E. and Garcia-Fraile P. (2017). Plant probiotic bacteria: solutions to feed the world. AIMS Microbiology , 3(3): 502-524. http://www.aimspress.com/article/10.3934/microbiol.2017.3.502
• Brígido, C., van Tuinen, D., Brito, I., Alho, L., Goss, M. J. and Carvalho, M. (2017). “Management of the biological diversity of AM fungi by combination of host plant succession and integrity of extraradical mycelium”. Soil Biology and Biochemistry , 112, 237-247.https://www.sciencedirect.com/science/article/pii/S0038071717305011
• Hudson, L. N., Newbold, T., Contu, S., Hill S. L. L., et al. “The database of the PREDICTS (Projecting Responses of Ecological Diversity In Changing Terrestrial Systems) project (2017)”. Ecology and Evolution 7(1):145-188. http://hdl.handle.net/10174/21869
• da-Silva, R., Alexandre, A., Brígido, C. and Oliveira, S. (2017) Can stress response genes be used to improve the symbiotic performance of rhizobia? AIMS Microbiology, 3:3, 354-382.http://www.aimspress.com/article/10.3934/microbiol.2017.3.365
• Brígido, C., Glick, B. R. and Oliveira, S. (2017) "Survey of Plant Growth-Promoting Mechanisms in Native Portuguese Chickpea Mesorhizobium  Isolates", Microbial Ecology , 73 :4, 900-915. 
    http://link.springer.com/article/10.1007/s00248-016-0891-9
• Laranjo, M., Alexandre, A., and Oliveira, S. (2017) "Global transcriptional response to salt shock of the plant microsymbiont Mesorhizobium loti MAFF303099", Research in Microbiology , 168 :1, 55-63.
  http://www.sciencedirect.com/science/article/pii/S0923250816300791
• Serrano J., Shahidian S., Silva J. M., Sales-Baptista E., Oliveira I. F., Castro J. L., Pereira A., Abreu M. C., Machado E.& Carvalho M. (2017): Tree influence on soil and pasture: contribution of proximal sensing to pasture productivity and quality estimation in montado ecosystems, International Journal of Remote Sensing, DOI: 10.1080/01431161.2017.1404166https://www.tandfonline.com/doi/full/10.1080/01431161.2017.1404166

2016

• Serrano J. M., Shahidian S., Marques J. S. and Carvalho M. (2016) Monitoring of soil organic carbon over 10 years in a Mediterranean silvo-pastoral system: potential evaluation for differential management. Precision Agric., 17: 274–295.https://link.springer.com/article/10.1007/s11119-015-9419-4
•  Paço, A., Brígido, C., Alexandre, A., Mateos, P.F., Oliveira, S. (2016) "The symbiotic performance of chickpea rhizobia can be improved by additional copies of the clpB  chaperone gene", PLoS ONE  11 (2): e0148221. doi:10.1371/journal.pone.0148221.http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0148221
• Nascimento F. X., Brígido C., Glick B. R., and Rossi M. J. (2016). The Role of Rhizobial ACC Deaminase in the Nodulation Process of Leguminous Plants. International Journal of Agronomy. vol. 2016, Article ID 1369472, 9 pages.https://www.hindawi.com/journals/ija/2016/1369472/

2015

• Alho L., Carvalho M., Brito I. and Goss M. J. (2015) “The effect of arbuscular mycorrhiza fungal propagules on the growth of subterranean clover (Trifolium subterraneum  L.) under Mn toxicity in ex situ  experiments”. Soil Use and Management , 31, 337–344. http://hdl.handle.net/10174/15937
• - Carvalho M., Brito I., Alho L. and Goss M. J. (2015) “Assessing the progress of colonization by arbuscular mycorrhiza of four plant species under different temperature regimes”. Journal of Plant Nutrition and Soil Science, 178, 515–522.http://hdl.handle.net/10174/14593
• Valverde P., Carvalho M., Serralheiro R., Maia R., ramos V. e Oliveira B. (2015) Climate change impacts on rainfed agriculture in the Guadiana riverbasin (Portugal). Agricultural Water Management 150 (2015) 35–45.https://www.sciencedirect.com/science/article/pii/S0378377414003692
• Valverde P., Serralheiro R., Carvalho M., Maia R., Oliveira B. and Ramos V. (2015) Climate change impacts on irrigated agriculture in the Guadiana riverbasin (Portugal). Agricultural Water Management 152: 17–30.https://www.sciencedirect.com/science/article/pii/S0378377414003989
• Carvalho, M., Serralheiro, R., Corte-Real, J. and Valverde, P. (2015) Implications of climate variability and future trends on wheat production and crop technology adaptations in southern regions of Portugal. Water Utility Journal 9: 13-18, 2015.http://www.ewra.net/wuj/pdf/WUJ_2015_09_02.pdf
•  Maia, R., Vivas, E., Serralheiro R. and Carvalho M. (2015) Socioeconomic Evaluation of Drought Effects. Main Principles and Application to Guadiana and Algarve Case Studies. Water Resource Management, 29: 575–588.https://link.springer.com/article/10.1007/s11269-014-0883-9

2014

•  Laranjo, M., Alexandre, A., and Oliveira, S. (2014) "Genes commonly involved in acid tolerance are not overexpressed in the plant microsymbiont Mesorhizobium loti  MAFF303099 upon acidic shock", Applied Microbiology and Biotechnology , 98 :16, 7137-7147.http://link.springer.com/article/10.1007/s00253-014-5875-4
• Alexandre, A., Laranjo, M., and Oliveira, S. (2014) "Global transcriptional response to heat shock of the legume symbiont Mesorhizobium loti  MAFF303099 comprises extensive gene downregulation", DNA Research , 21 :2, 195-206. http://dnaresearch.oxfordjournals.org/content/21/2/195.abstract
• Laranjo, M., Alexandre, A. and Oliveira, S. (2014) "Legume growth-promoting rhizobia: an overview on the Mesorhizobium  genus", Microbiological Research , 169 :1, 2-17.http://www.sciencedirect.com/science/article/pii/S0944501313001651  
  Brito I., Carvalho M., Alho L. and Goss M. J. (2014) “Managing arbuscular mycorrhizal fungi for bioprotection: Mn toxicity”. Soil Biology and Biochemistry , 68,78-84. http://hdl.handle.net/10174/9077
• Hudson, L. N., Newbold, T., Contu, S., Hill S. L. L. et al. (2014) “The PREDICTS database: a global database of how local terrestrial biodiversity responds to human impacts”. Ecology and Evolution , 4, 4701–4735.http://hdl.handle.net/10174/14113
• Carvalho, M. and Lourenço, E. (2014) Conservation Agriculture – A Portuguese Case Study. Review article. Journal of Agronomy and Crop Sciences: 317-324 ISSN 0931-2250https://onlinelibrary.wiley.com/doi/abs/10.1111/jac.12065

2013

• Brito I., Goss M. J. and Carvalho M. (2013) “Soil and weed management for enhancing arbuscular mycorrhiza colonisation of wheat”. Soil Use and Management , 29, 540-546. http://hdl.handle.net/10174/8714
• Brígido, C., Nascimento, F. X., Duan, J., Glick, B. R. and Oliveira, S. (2013) "Expression of an exogenous 1-aminocyclopropane-1-carboxylate deaminase gene in Mesorhizobium  spp. reduces the negative effects of salt stress in chickpea", FEMS Microbiology Letters , 349 :1, 46-53. http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12294/abstract
• Alexandre, A. and Oliveira, S. (2013) "Response to temperature stress in rhizobia", Critical Reviews in Microbiology , 39 :3, 219-228. http://informahealthcare.com/doi/abs/10.3109/1040841X.2012.702097
• Brígido, C. and Oliveira, S. (2013) "Most acid-tolerant chickpea mesorhizobia show induction of major chaperone genes upon acid shock", Microbial Ecology , 65 :1, 145-153. http://www.springerlink.com/content/87k67g8364u865n0/
• Calado. J.M.G., Basch, G., Barros, J.F.C. and Carvalho, M. (2013). Weed emergence as affected by soil disturbance and moisture in a controlled environment. Plant Protection Quarterly, Vol. 28, 1: 6-11.https://search.informit.com.au/documentSummary;dn=453473177525689;res=E-LIBRARY
• Brito I., Carvalho M., Alho L., Caseirio M. and Goss M. J. (2013) Practical exploitation of mycorrhizal fungi in agricultural systems. Aspects of Applied Biology 121, 25:30https://dspace.uevora.pt/rdpc/bitstream/10174/9812/1/6%20BRITO.pdf

2012

• Brito I., Goss M. J., Carvalho M., Chatagnier O. and Van Tuinen D. (2012) “Impact of tillage system on arbuscular mycorrhiza fungal communities in the soil under Mediterranean conditions”. Soil & Tillage Research,  121 , 63–67.http://hdl.handle.net/10174/6828
• Brito I., Goss M. J. and Carvalho M. (2012) “Effect of tillage and crop on arbuscular mycorrhiza colonisation of winter wheat and triticale under Mediterranean conditions”. Soil Use and Management , 28, 201-208.http://hdl.handle.net/10174/6829
• Brígido, C., Robledo, M., Menéndez, E., Mateos, P. F. and Oliveira, S. (2012) "A ClpB Chaperone Knockout Mutant of Mesorhizobium ciceri  Shows a Delay in the Root Nodulation of Chickpea Plants". Molecular Plant Microbe Interactions , 25 :12, 1594-1604.http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-05-12-0140-R?journalCode=mpmi
• Brígido, C., Alexandre, A. and Oliveira, S. (2012) "Transcriptional analysis of major chaperone genes in salt-tolerant and salt-sensitive mesorhizobia", Microbiological Research , 167 :10, 623-629. http://www.sciencedirect.com/science/article/pii/S094450131200016X
• Nascimento, F., Brígido, C., Glick, B. R. and Oliveira, S. (2012) "ACC deaminase genes are conserved among Mesorhizobium  species able to nodulate the same host plant". FEMS Microbiology Letters , 336 :1, 26-37. 
  http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2012.02648.x/abstract
• Laranjo, M., Young, J. P. W. and Oliveira, S. (2012) "Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium  species". Systematic and Applied Microbiology , 35 :6, 359-367.
• http://dx.doi.org/10.1016/j.syapm.2012.06.002
• Basch, G., Calado, J.M.G., Barros, J.F.C., and Carvalho, M. (2012). Impact of soil tillage and land use on soil organic carbon decline under Mediterranean conditions. Agrociencia, (Special Issue), 175-182, ISSN 1510-0839http://www.fagro.edu.uy/agrociencia/index.php/directorio/article/view/685
• Nascimento, F., Brígido, C., Glick, B.R., Oliveira, S., Alho, L. (2012) “Mesorhizobium ciceri  LMS-1 expressing an exogenous ACC deaminase increases its nodulation abilities and chickpea plant resistance to soil constraints”. Letters in Applied Microbiology , 55 :1, 15-21.http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2012.03251.x/abstract
• Nascimento, F., Brígido, C., Alho, L., Glick, B. R. and Oliveira, S. (2012) "Enhanced chickpea growth-promotion ability of a Mesorhizobium  strain expressing an exogenous ACC deaminase gene". Plant and Soil , 353: 1-2, 221-230. http://www.springerlink.com/content/8g83510828408865/  

2011

• Brito I., Carvalho M. & Goss M.J. (2011) “Summer survival of arbuscular mycorrhiza extraradical mycelium and the potential for its management through tillage options in Mediterranean cropping systems”. Soil Use and Management , 27, 350-356. http://hdl.handle.net/10174/3506
• Laranjo, M. and Oliveira, S. (2011) "Tolerance of Mesorhizobium  type strains to different environmental stresses". Antonie van Leeuwenhoek , 99 :3, 651-662.http://www.springerlink.com/content/x3q68552ur737354/abstract 
• Alexandre, A. and Oliveira, S. (2011) "Most heat-tolerant rhizobia show high induction of major chaperone genes upon stress", FEMS Microbiology Ecology , 75 :1, 28-36. http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2010.00993.x/abstract  
• Serrano, J.M.; Peça J.O.; Silva J.R.M.; Shahidian S. and Carvalho M. (2011) Phosphorus dynamics in permanent pastures: differential fertilizing and the animal effect. Nutr Cycl. Agroecosyst. 90:63-74. DOI 0.1007/s10705-010-9412-2https://link.springer.com/article/10.1007%2Fs10705-010-9412-2

2010

• Calado M.G., Basch G. and Carvalho M. (2010) Weed management in no-till winter wheat (Triticum aestivum L.) Crop Protection 29, issue 1, 1-6.https://www.sciencedirect.com/science/article/pii/S0261219409002488
• Peça J.O., Serrano J.M., Pinheiro A., Carvalho M., Nunes M., Ribeiro L., Santos F. (2010) Speed advice for power efficient drawbar work. Journal of Terramechanics 47: 55-61https://www.sciencedirect.com/science/article/pii/S0022489809000913

Previous years

• Alexandre, A., Brígido, C., Laranjo, M., Rodrigues, S. and Oliveira, S. (2009) "Survey of chickpea rhizobia diversity in Portugal reveals the predominance of species distinct from Mesorhizobium ciceri  and Mesorhizobium mediterraneum ", Microbial Ecology , 58 :4, 930-941.http://www.springerlink.com/content/07027073383qw737/?p=b700573d0d0d446bbdb21704495c4216&pi=20  
• Calado J.M.G., Basch G. and Carvalho M. (2009) Weed emergence as influenced by soil moisture and air temperature. J. Pest Sci. 82:81-88https://link.springer.com/article/10.1007%2Fs10340-008-0225-x
• Barros, J. F. C., Basch, G., Freixial, R. & Carvalho, M. (2009). Effect of reduced doses of mesosulfuron + iodosulfuron to control weeds in no-till wheat under Mediterranean conditions. Spanish Journal of Agricultural Research, 7 (4): 905-912http://revistas.inia.es/index.php/sjar/article/view/1104
• Alexandre, A., Laranjo, M., Young, J. P. W. and Oliveira, S. (2008) "dnaJ  is a useful phylogenetic marker for alphaproteobacteria" International Journal of Systematic and Evolutionary Microbiology , 58 :12, 2839-2849.
  http://ijs.sgmjournals.org/cgi/content/abstract/58/12/2839
• Laranjo, M., Alexandre, A., Rivas, R., Velázquez, E., Young, J. P. W. and Oliveira, S. (2008) "Chickpea rhizobia symbiosis genes are highly conserved across multiple Mesorhizobium  species" FEMS Microbiology Ecology , 66 :2, 391-400.http://www3.interscience.wiley.com/journal/121409776/abstract
• Serrano, João; Peça, José; Pinheiro, Anaclet; Carvalho, Mário (2008) Evaluation of the energy requirements in tractor-disc harrow systems. Spanish Journal of Agricultural Research, vol 62(2): 205-209http://hdl.handle.net/10174/1598
• Silva J.R.M., Peça J.O., Serrano J.M. Carvalho M. & Palma P.M. (2008) Evaluation of spatial and temporal variability of pasture based on topography and the quality of the rainy season. Precision Agric. 9: 209-229https://link.springer.com/article/10.1007%2Fs11119-008-9066-0
• Barros, J. F. C., Basch, G. & Carvalho, M. (2008). Effect of reduced doses of a post-emergence graminicide to control Avena sterilis L. and Lolium rigidum G. in no-till wheat under Mediterranean environment. Crop Protection, vol. 27 (6): 1031-1037.https://www.sciencedirect.com/science/article/pii/S0261219407003304
• Brígido, C., Alexandre, A., Laranjo, M., Oliveira, S. (2007) "Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum  L.)" Letters in Applied Microbiology , 44 :2, 168-174.
  http://www.blackwell-synergy.com/doi/abs/10.1111/j.1472-765X.2006.02061.x
• Rivas, R., Laranjo,M., Mateos, P.F., Oliveira, S., Martínez-Molina, E. and Velázquez, E. (2007) "Strains of Mesorhizobium amorphae  and M. tianshanense carrying symbiotic genes of common chickpea endosymbiotic species constitute a novel biovar (ciceri) able to nodulate Cicer arietinum  " Letters in Applied Microbiology , 44 :4, 412-418.http://www.blackwell-synergy.com/doi/abs/10.1111/j.1472-765X.2006.02086.x
• Serrano, João; Peça, José; Pinheiro, Anacleto; Carvalho, Mário; Silva, José (2007) Tractor Energy Requirements in Disc Harrow Systems. Biosystems Engineering, vol 98, issue 3: 286-296 https://www.sciencedirect.com/science/article/pii/S1537511007001985
• Barros, J. F. C., Basch, G. & Carvalho, M. (2007). Effect of reduced doses of a post-emergence herbicide to control grass and broad-leaved weeds in no – till wheat under Mediterranean conditions. Crop Protection, vol. 26 (10): 1538-1545.https://www.sciencedirect.com/science/article/pii/S0261219407000336
• Serrano, João; Peça, José; Pinheiro, Anacleto; Carvalho, Mário; Silva, José (2007) Tractor Energy Requirements in Disc Harrow Systems. Biosystems Engineering, vol 98, issue 3: 286-296 https://www.sciencedirect.com/science/article/pii/S1537511007001985
• Alexandre, A., Laranjo, M. and Oliveira, S. (2006) "Natural populations of chickpea rhizobia evaluated by antibiotic resistance profiles and molecular methods" Microbial Ecology , 51 :1, 128-136.
• http://www.springerlink.com/content/p115188161m5x7l6/
• Rodrigues, C., Laranjo, M. and Oliveira, S. (2006) "Effect of heat and pH stress in the growth of chickpea mesorhizobia" Current Microbiology , 53 :1, 1–7.
  http://www.springerlink.com/content/00g7wu1vv5u75002/
• Carvalho M. (2006) Effects of direct drilling on the fertilization of cereals, Options Mediterranéennes, serie A: Séminaires Méditerranées, number 69, 63-68.http://om.ciheam.org/om/pdf/a69/06600086.pdf
• Barros, J. F. C., Basch, G. ad Carvalho, M. (2005). Effect of reduced doses of a post-emergence graminicide mixture to control Lolium rigidum G. in winter wheat under direct drilling in Mediterranean environment. Crop Protection, vol. 24, nº 10: 880-887.https://www.sciencedirect.com/science/article/pii/S0261219405000505
•  Laranjo, M., Machado, J., Young, J.P.W., Oliveira, S. (2004) “High diversity of chickpea Mesorhizobium  species isolated in a Portuguese agricultural region” FEMS Microbiology Ecology , 48 :1,101-107.
  http://www.blackwell-synergy.com/doi/abs/10.1016/j.femsec.2003.12.015
• Barros, J. F. C., Carvalho, M. and Basch, G. (2004). Response of sunflower (Helianthus annuus L) to sowing date and plant density under Mediterranean conditions. European Journal of Agronomy, vol. 21, nº 3: 347 – 356.https://www.sciencedirect.com/science/article/pii/S1161030103001096
• Barros, J. F. C., Carvalho, M. and Basch, G. (2004). Response of sunflower (Helianthus annuus L) to sowing date and plant density under Mediterranean conditions. European Journal of Agronomy, vol. 21, nº 3: 347 – 356.https://www.sciencedirect.com/science/article/pii/S1161030103001096
• Serrano, João M.; Peça, J. O.; Pinheiro, A.; Carvalho, M.; Nunes, M.; Ribeiro, L.; Santos, F. (2003) -The effect of Gang Angle of Offset Disc Harrows on Soil Tilth, Work Rate and Fuel Consumption. Biosystems Engineering, Volume 84(2): 171-17https://www.sciencedirect.com/science/article/pii/S1537511002002611
• Laranjo, M., Branco, C., Soares, R.,  Alho, L., Carvalho, M. and Oliveira, S. (2002) “Comparison of chickpea rhizobia isolates from diverse Portuguese natural populations based on symbiotic effectiveness and DNA fingerprint” Journal of  Applied Microbiology  92 :6, 1043-50.http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2672.2002.01615.x
• Laranjo, M., Rodrigues, R., Alho, L., and Oliveira, S. (2001) "Rhizobia of chickpea from Southern Portugal: symbiotic efficiency and genetic diversity" Journal of Applied Microbiology ,  90 :4, 662-667.http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2672.2001.01295.x